ABSTRACT
Various concentrations of chilli extract were tested in 2 stages on the growth of a human buccal mucosa fibroblast cell line. Firstly, concentrations of 25, 75, 150 and 300 micrograms/ml were tested on the cell line for 6 days followed by a repeat which included concentrations of 400 and 500 micrograms/ml. In the latter, cell growth was monitored for 18 days. From day 3 (72 hr) suppression of cell growth was evident with concentrations 300-500 micrograms/ml. Total cell death occurred at 16 days with 300 micrograms/ml and at 6 days with 400-500 micrograms/ml. With the lower concentrations, 25-150 micrograms/ml, the daily counts were lower than the control but the difference was not statistically significant. Growth continued unabated. It is therefore concluded that cytopathic effect of chilli extract to fibroblasts is concentration dependent.
Subject(s)
Capsicum/chemistry , Cell Division/drug effects , Cell Line , Fibroblasts/cytology , Humans , Mouth Mucosa/cytology , Plant Extracts/pharmacology , Plants, MedicinalABSTRACT
Lesöes displásicas corticais (LDC) estäo frequentemente associadas a epilepsias de difícil controle. Nós descrevemos os correlatos eletrográficos deste alto grau de epileptogenicidade, caracterizado por descargas epileptógenas rítmicas, contínuas ou quase-contínuas, registradas diretamente das LDC durante eletrocorticografia trans-operatória. Esta atividade ictal ou descargas epileptogênicas contínuas (AI/DEC) manifestava-se por um de três padröes: (a) crises eletrográficas repetidas; (b) surtos de polipontas recorrentes; ou (c) espículas rítmicas contínuas ou quase-contínuas. Um ou mais destes padröes estava presente em 23 de 34 pacientes (67 por cento ) com epilepsia partial de difícil controle associada a LDC. Esta AI/DEC era geralmente mais localizada, assim contrastando com a atividade interictal ao eletrocorticograma, que era mais difusa. Nós concluimos que LDC säo altamente e intrinsicamente epileptogênicas e discutimos mecanismos possivelmente relacionados a esta epileptogenicidade intrínsica